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Class 12 Tuition > Learn Class XI-XII Tuition (PUC) > How do stains used for light microscopy compare with those...
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How do stains used for light microscopy compare with those used for electron microscopy?

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Stains used for light microscopy are colored molecules that bind to cell componets, affecting the light oassing through. The stains used for electron microscopy involve heavy metals taht affect the beams of electrons passign through.
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In light microscopy main source is light/illumination. In Electron microscopy main source is electrons.
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Light microscopy involves coloured molecules and uses light as source. Whereas in Electron microscopy electric charges (electrons in particular) are obtained upon bombardment.
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For light microscopy, the main source spectrum of the rainbow. A stain is used to absorb all the different colors except the target colour. For instance in a red stain , it absorbs all other colors except red. The red light reaches to our eye. In electron microscopy, the source of illumination is electrons....
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For light microscopy, the main source spectrum of the rainbow. A stain is used to absorb all the different colors except the target colour. For instance in a red stain , it absorbs all other colors except red. The red light reaches to our eye. In electron microscopy, the source of illumination is electrons. The pattern of the electrons that passes is making the image . So sample must conduct electrons. So a stain comprising of heavy metal such like uranium is used for this puropose. read less
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For your knowledge, at the very fundamental level there is a difference that optical light microscopy involves optical lenses using refraction, a wave property and electron microscope makes use of electrostatic lenses which makes use of deflection of individual electrons in an electron beam as individual,...
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For your knowledge, at the very fundamental level there is a difference that optical light microscopy involves optical lenses using refraction, a wave property and electron microscope makes use of electrostatic lenses which makes use of deflection of individual electrons in an electron beam as individual, classical particles. If photon picture used to explain optical microscope's action, we say the uncertainty principle applied to each photon helps focusing of each photon! read less
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A wave property and electron microscope makes use of electrostatic lenses which makes use of deflection of individual electrons in an electron beam as individual, classical particles.
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