Class is for BTech students of BTech Biotechnology Engineering. All important topics in the genomics and proteomics will be covered in the session. The students can also ask doubts during the session. It will cover 

UNIT I: Introduction to Genomics and Proteomics: Introduction – Organization and structure of genomes, Genome size, Sequence complexity, Introns and Exons, Genome structure in viruses and prokaryotes, Isolation of Chromosomes, chromosome micro dissection, Retrofitting. Introduction to Proteomics – The Proteome, Mining proteomes, Bridging Genomics and Proteomics. Proteomics and the new biology. UNIT II: Gene Identification and Expression: Genome annotation, traditional routes of gene identification, detecting open-reading Frames, software programs for finding genes, Identifying the function of a new gene, gene ontology, overview of comparative genomics, Protein structural genomics, determining gene function by sequence comparison and through conserved protein structure Global expression profiling – Introduction, traditional approaches to expression profiling, Analysis of RNA expression, applications of genome analysis and genomics. UNIT III: Analysis of Proteomes I: Analysis of proteomes - Two-dimensional polyacrylamide gel electrophoresis, Sample Preparation, Solubilization, Reduction, Resolution, Reproducibility of 2-DEDetecting proteins in polyacrylamide gels, Image analysis of 2-DE gels. UNIT IV: Analysis of Proteomes II: Mass spectrometry based methods for protein identification- De novo sequencing using mass spectrometric data- Correlative mass spectrometric based identification strategies, 2-DE gel electrophoresis coupled with mass spectrometry, Micro array techniques- Types of micorarrays, Designing a microarray experiment, Microarray Technology in Treating Disease. UNIT V: Applications of Genomics and Proteomics Analysis: Analysis of Genomes – Human, Mouse, Plasmodium falsiparum,Saccharomyces cerevisiae, Mycobacterium tuberculosis. Application of proteome analysis- drug development and toxicology, Pharmaceutical Applications, Proteomics in drug Discovery in human, phage antibodies as tools, Glycobiology and Proteomics in plant genetics and breeding.