UPSC Chemistry

Compare the features of different types of detectors used in HPLC system:

1. HPLC is a separation technique in which liquid is used to force the sample at high pressure through a column packed with stationary phase in order to identify and/or quantify the components.

2. A typical HPLC system consists of a pump (consistent flow rate is key), an injector or autosampler, and at least one detector.

3. Also, HPLC systems often use a column oven to adjust and control the temperature of the separation step. A variety of detector types are available, including UV-VIS, refractive index (RI), photo diode array, and fluorescence. 

4. Biochemistry, biology, and the pharmaceutical industry all make use of the high resolution and fast separation possible with an HPLC system.