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UPSC Chemistry

Nidhi T.
05/07/2017 0 0

Compare the features of different types of detectors used in HPLC system:

  1. HPLC is a separation technique in which liquid is used to force the sample at high pressure through a column packed with stationary phase in order to identify and/or quantify the components.

  2. A typical HPLC system consists of a pump (consistent flow rate is key), an injector or autosampler, and at least one detector.

  3. Also, HPLC systems often use a column oven to adjust and control the temperature of the separation step. A variety of detector types are available, including UV-VIS, refractive index (RI), photo diode array, and fluorescence. 

  4. Biochemistry, biology, and the pharmaceutical industry all make use of the high resolution and fast separation possible with an HPLC system. 

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